Journal: Applied and Environmental Microbiology

Article Title: Nitrogen Cycle Evaluation (NiCE) Chip for Simultaneous Analysis of Multiple N Cycle-Associated Genes

doi: 10.1128/AEM.02615-17

Figure Lengend Snippet: Pipeline of the NiCE chip assay. (a) Quantitative PCR is performed by using a 48.48 AccessArray (AA) chip with EvaGreen chemistry. (b) Recovered PCR amplicons are tagged with an Illumina index and adaptors (Illumina) and used for MiSeq 300-bp paired-end sequencing.

Article Snippet: The objectives of this study are to (i) simultaneously quantify multiple N cycle functional genes (Fig. S1) as well as taxon-specific 16S rRNA gene markers by using microfluidic qPCR chip technology (nitrogen cycle evaluation [NiCE] chip), (ii) identify key microbes contributing to the N cycle by sequencing the PCR amplicons recovered from the NiCE chip , and (iii) apply the NiCE chip amplicon sequencing approach for analysis of mixed-culture samples and environmental samples. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 Pipeline of the NiCE chip assay. (a) Quantitative PCR is performed by using a 48.48 AccessArray (AA) chip with EvaGreen chemistry. (b) Recovered PCR amplicons are tagged with an Illumina index and adaptors (Illumina) and used for MiSeq 300-bp paired-end sequencing.

Techniques: Real-time Polymerase Chain Reaction, Sequencing